CD4 T cell immunity to Salmonella is transient in the circulation

NG Peres; N Wang; P Whitney; S Engel; MM Shreenivas; I Comerford; DM Hocking; AB Erazo; I Förster; A Kupz; T Gebhardt; SR McColl; SJ McSorley; S Bedoui; RA Strugnell

Journal article

CD4 T cell immunity to Salmonella is transient in the circulation

NG Peres, N Wang, P Whitney, S Engel, MM Shreenivas, I Comerford, DM Hocking, AB Erazo, I Förster, A Kupz, T Gebhardt, SR McColl, SJ McSorley, S Bedoui, RA Strugnell

Plos Pathogens | Published : 2021

DOI: 10.1371/journal.ppat.1010004

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Abstract

While Salmonella enterica is seen as an archetypal facultative intracellular bacterial pathogen where protection is mediated by CD4+ T cells, identifying circulating protective cells has proved very difficult, inhibiting steps to identify key antigen specificities. Exploiting a mouse model of vaccination, we show that the spleens of C57BL/6 mice vaccinated with live-attenuated Salmonella serovar Typhimurium (S. Typhimurium) strains carried a pool of IFN-γ+ CD4+ T cells that could adoptively transfer protection, but only transiently. Circulating Salmonella-reactive CD4+ T cells expressed the liver-homing chemokine receptor CXCR6, accumulated over time in the liver and assumed phenotypic chara..

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University of Melbourne Researchers

Nancy Wang Author

Sven Engel Author

Thomas Gebhardt Author

Sammy Bedoui Author

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Awarded by National Institute of Allergy and Infectious Diseases

Funding Acknowledgements

N.G.P., N.W., P.W., A.B.E., S.B. and R.A. S. were supported by National Health and Medical Research Council (Australia) (NHMRC) Program Grant 1092262 and 1059937. https://www.nhmrc.gov.au.N.W.and R.A.S. were supported by Australian Research Council (ARC) Discovery Project DP200103110. https://www.arc.gov.au.S.J.M was supported from the National Institute of Allergy and Infectious Diseases (Grants AI056172 and AI139410). https://www.niaid.nih.gov.I.F.was supported by the Deutsche Forschungsgemeinschaft (DFG) through GRK 2168. I.F. is a member of the DFG-funded Cluster of Excellence ImmunoSensation. https://www.dfg.de.The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.